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HPLC of monolayer-protected Gold clusters with baseline separation




The properties of ultrasmall metal nanoparticles (ca. 10–200 metal atoms), or monolayer-protected metal clusters (MPCs), drastically depend on their atomic structure. For systematic characterization and application, assessment of their purity is of high importance. Currently, the gold standard for purity control of MPCs is mass spectrometry (MS). Mass spectrometry, however, cannot always detect small impurities; MS of certain clusters, for example, ESI-TOF of Au40(SR)24, is not successful at all. We here present a simple reversed-phase HPLC method for purity control of a series of small alkanethiolate-protected gold clusters. The method allows the detection of small impurities with high sensitivity. Linear correlation between alkyl chain length of Au25(SC_n H_(2n+1))18 clusters (n = 6, 8, 10, 12) and their retention time was noticed.

Author(s): Knoppe, S. and Vogt, P.
Journal: Analytical Chemistry
Volume: 91
Pages: 1603
Year: 2019
Month: December
Day: 19

Department(s): Micro, Nano, and Molecular Systems
Bibtex Type: Article (article)

DOI: https://doi.org/10.1021/acs.analchem.8b05064
URL: https://pubs.acs.org/doi/10.1021/acs.analchem.8b05064


  title = {HPLC of monolayer-protected Gold clusters with baseline separation},
  author = {Knoppe, S. and Vogt, P.},
  journal = {Analytical Chemistry},
  volume = {91},
  pages = {1603},
  month = dec,
  year = {2019},
  url = {https://pubs.acs.org/doi/10.1021/acs.analchem.8b05064},
  month_numeric = {12}